Medhat Mahmoud PostDoc
Research
I am a computational Biologist focusing on method developing for sequencing applications.
In general, my interests focus on three key areas:
- Sequence Analysis:
- Method development to analyze Next Generation Sequencing (NGS) data
- Detection of Structural Variations
- Assessment of the variability of genomes
- High performance computing:
- Programming on GPU Graphics Cards (CUDA, OpenCL)
- Programming on CPUs (SSE, AVX)
- Programming on Grid Engine (SGE, Slurm)
Education
- M.Sc In Genetic Enginerring and Biotechnology, Bioinformatics department, 2011
- Ph.D in Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań Poland 2018
Work experience
- 08/2007-01/2008: Internship
- The European Molecular Biology Laboratory
- Duties included: Method development for cryo electron microscopy
- Supervisor: Achilleas Frangakis
- 11/2008-12/2012: PhD student
- Cibiv - Max F. Perutz Laboratories
- Duties included: Method development for NGS and SVs detection
- Supervisor: Arndt von Haeseler
- 01/2013-12/2014: PostDoc
- Cibiv - Max F. Perutz Laboratories
- Duties included: Method development for NGS and SVs detection
- Supervisor: Arndt von Haeseler
- 12/2014-12/2015: Computational Sci. Analyst I
- Simons Center for Quantitative Biology - Cold Spring Harbor Laboratories
- Duties included: Method development for NGS and SVs detection
- Supervisor: Michael C. Schatz
- 12/2015-04/2017: PostDoc
- Department of Computer Science - Johns Hopkins University
- Duties included: Method development for NGS and SVs detection
- Supervisor: Fritz J Sedlazeck
- 09/2018-present: PostDoc
- HGSC, Baylor College of Medicine
- Duties included: Method development for NGS and SVs detection
- Supervisor: Michael C. Schatz
Publications
Advanced Methylome Analysis after Bisulfite Deep Sequencing: an Example in Arabidopsis
H.Q. Dinh, M. Dubin*, F.J. Sedlazeck*, N. Lettner, O. Mittelsten Scheid, and A. von Haeseler (2012). "Advanced Methylome Analysis after Bisulfite Deep Sequencing: an Example in Arabidopsis." Genome Research. 22, 1468-1476.
Benefit-of-doubt (BOD) scoring: a sequencing-based method for SNP candidate assessment from high to medium read number data sets
F.J. Sedlazeck, P. Talloji, A. von Haeseler, and A. Bachmair (2012). "Benefit-of-doubt (BOD) scoring: a sequencing-based method for SNP candidate assessment from high to medium read number data sets." Genomics. 101, 204-209.
Updating benchtop sequencing performance comparison
S. Jünemann, F.J. Sedlazeck, K. Prior, A. Albersmeier, U. John, J. Kalinowski, A. Mellmann, A. Goesmann, A. von Haeseler, J. Stoye, and D. Harmsen (2013). "Updating benchtop sequencing performance comparison." Nature Biotechnology. 31, 294-296.
NextGenMap: fast and accurate read mapping in highly polymorphic genomes
F.J. Sedlazeck∗, P. Rescheneder∗, and A. von Haeseler (2013). "NextGenMap: fast and accurate read mapping in highly polymorphic genomes." Bioinformatics. 29, 2790-2791.
Adenosine deaminases that act on RNA induce reproducible changes in abundance and sequence of embryonic miRNAs
C. Vesely, S. Tauber, F.J. Sedlazeck, M. Tajaddod,A. von Haeseler, M.F. Jantsch (2014). "ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain." Nucleic Acids Research. 42, 2155-12168.
Decreased expression of endogenous feline leukemia virus in cat lymphomas: a case control study
M. Krunic, R. Ertle, B. Hagen, F.J. Sedlazeck, R. Hofmann- Lehmann, A. von Haeseler and D. Klein (2012). "Decreased expression of endogenous feline leukemia virus in cat lymphomas: a case control study." BMC Veterinary Research. 11, 90.
Ectodysplasin signalling genes and phenotypic evolution in sculpins (Cottus)
J. Cheng, F.J. Sedlazeck, J. Altmüller, A.W. Nolte (2015). "Ectodysplasin signalling genes and phenotypic evolution in sculpins (Cottus)." Proceedings B. 282, 20150746.
The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways
M. Tscherner, F. Zwolanek, S. Jenull, F.J. Sedlazeck, I.E. Frohner, N. Chauhan, A. von Haeseler and K. Kuchler (2012). "The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways." PLOS Pathogen. 11, e1005218.
Teaser: Individualized benchmarking and optimization of read mapping results for NGS data
M. Smolka, P. Rescheneder, M.C. Schatz, A. von Haeseler, F.J. Sedlazeck (2015). "Teaser: Individualized benchmarking and optimization of read mapping results for NGS data." Genome Biology. 16, 235.
The pineapple genome and the evolution of CAM photosynthesis
R. Ming, R. VanBuren, C.M. Wai, H. Tang, M.C. Schatz, J.E. Bowers, E. Lyons, M. Wang, J. Chen, E. Biggers, J. Zhang, L. Huang, L. Zhang, W. Miao, J. Zhang, Z. Ye, C. Miao, Z. Lin, H. Wang, H. Zhou, W.C. Yim, H.D. Priest, C. Zheng, M. Woodhouse, P.P. Edger, R. Guyot, H. Guo, H. Guo, G. Zheng, R. Singh, A. Sharma, X. Min, Y. Zheng, H. Lee, J. Gurtowski, F.J. Sedlazeck, A. Harkess, M.R. McKain, Z. Liao, J. Fang, J. Liu, X. Zhang, Q. Zhang, W. Hu, Y. Qin, K. Wang, L. Chen, N. Shirley, Y. Lin, L. Liu, A.G. Hernandez, C.L. Wright, V. Bulone, G.A. Tuskan, K. Heath, F. Zee, P.H. Moore, R. Sunkar, J. H. Leebens-Mack, T. Mockler, J.L. Bennetzen, M. Freeling, D. Sankoff, A.H. Paterson, X. Zhu, X. Yang, J.A.C. Smith, J.C. Cushman, R.E. Paull, Q. Yu(2012). "The pineapple genome and the evolution of CAM photosynthesis," Nature Genetics. 47, 1435-1442.
Chromosomal- Level Assembly of the Asian Seabass Genome Using Long Sequence Reads and Multi-layered Scaffolding
S. Vij, H. Kuh, I. S. Kuznetsova, A. Komissarov, A. A.Yurchenko, P. V. Heusden, S. Singh, N. M. Thevasagayam, P.S.R. Sridatta, K. Purushothaman, J.M. Saju1, J. Jiang, S.K. Mbandi, M. Jonas, A.H.Y. Tong, S. Mwangi, D. Lau, S.Y. Ngoh, W.C. Liew, X. Shen, L.S. Hon, J.P. Drake, M. Boitano, R. Hall, J. Chin, R. Lachumanan, J. Korlach, V. Trifonov, M. Kabilov, A. Tupikin, D. Green, S. Moxon, T. Garvin, F.J. Sedlazeck, G.W. Vurture, G. Gopikrishna, V.K. K, T.H. Noble, V. Scaria, S. Sivasubbu, D.R. Jerry, S.J. O’Brien, M.C. Schatz, T. Dalmay, S. Turner, S. Lok, A. Christoffels, L. Orbán(2012). "Chromosomal- Level Assembly of the Asian Seabass Genome Using Long Sequence Reads and Multi-layered Scaffolding" Plos Genetics. 12(4): e1005954.
Phased diploid genome assembly with single-molecule real-time sequencing
C.S. Chin, P. Peluso, F.J. Sedlazeck, M. Nattestad, G.T. Con- cepcion, A. Clum, C. Dunn, R.O. Malley, R. Figueroa-Balderas, A. Morales-Cruz, G.R. Cramer, M. Delledonne, C. Luo, J.R. Ecker, D. Cantu, D.R. Rank, M.C. Schatz (2016) "Phased diploid genome assembly with single-molecule real-time sequencing." Nature Methods. 22, 1468-1476.
The genomic basis of circadian and circalunar timing adaptations in a midge
T.S. Kaiser, B. Poehn, D. Szkiba, M. Preussner, F.J. Sedlazeck, A. Zrim, T. Neumann, L. Nguyen, A.J. Betancourt, T. Hummel, H. Vogel, S. Dorner, F. Heyd, A. von Haeseler, K. Tessmar- Raible (2016). "The genomic basis of circadian and circalunar timing adaptations in a midge." Nature. 540, 69–73.
Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast
D.C. Jeffares, C. Jolly, M. Hoti, D. Speed, L. Shaw, C. Rallis, F. Balloux, C. Dessimoz*, J. Bähler*, F.J. Sedlazeck* (2017). "Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast." Nature Communications. 14061.
GenomeScope: fast reference-free genome profiling from short reads
G. Vurture*, F.J. Sedlazeck*, M. Nattestad, C. Underwood, H. Fang, J. Gurtowski, M.C. Schatz, (2017). "GenomeScope: fast reference-free genome profiling from short reads." Bioinformatics. 33 (14): 2202-2204.
DangerTrack: A scoring system to detect difficult-to-assess regions
I. Dolgalev*, , F.J. Sedlazeck* , B. Busby (2017). "DangerTrack: A scoring system to detect difficult-to-assess regions." F1000 Research. 6:443.
Copy number increases of transposable elements and protein coding genes in an invasive fish of hybrid origin
S. Dennenmoser, F.J. Sedlazeck, E. Iwaszkiewicz, X. Li, J. Alt- müller, A.W. Nolte (2017). "Copy number increases of transposable elements and protein coding genes in an invasive fish of hybrid origin." Molecular Ecology.
LRSim: A Linked-Reads Simulator generating insights for better genome partitioning.
R. Luo, FJ. Sedlazeck, CA. Darby, SM. Kelly, MC. Schatz (2017). "LRSim: a Linked Reads Simulator generating insights for better genome partitioning" Computational and Structural Biotechnology Journal.
Piercing the dark matter: bioinformatics of long-range sequencing and mapping.
FJ. Sedlazeck, H. Lee, CA. Darby, MC. Schatz (2018). "Piercing the dark matter: bioinformatics of long-range sequencing and mapping." Nature Genetics Reviews.
Accurate detection of complex structural variations using single-molecule sequencing.
FJ. Sedlazeck, P. Rescheneder, M. Smolka, H. Fang, M. Nattestad, A. Haeseler, MC. Schatz (2018). "Accurate detection of complex structural variations using single-molecule sequencing." Nature Methods.
Complex rearrangements and oncogene amplifications revealed by long-read DNA and RNA sequencing of a breast cancer cell line.
M. Nattestad, S. Goodwin, K. Ng, T. Baslan, FJ. Sedlazeck, P. Rescheneder, T. Garvin, H. Fang, J. Gurtowski, E. Hutton, E. Tseng, J. Chin, T. Beck, Y. Sundaravadanam, M. Kramer, E. Antoniou, J. McPherson, J. Hicks, WR. McCombie, MC Schatz. (2018). "Complex rearrangements and oncogene amplifications revealed by long-read DNA and RNA sequencing of a breast cancer cell line." Genome Research.
Talks
From CPU to GPU: An Application in bioinformatics
Talk at TU Munich, Department of Testing, Munich, Germany
NextGenMap: Using high throughput hardware for high throughput sequencing
Talk at Research Institute of Molecular Pathology (IMP), Vienna, Austria
Whole Genome Sequencing: Possibilities, Perspectives and Problems for the Analysis
Talk at Research Institute of Molecular Pathology (IMP), Vienna, Austria
Identifying wrongly mapped reads via majority vote of several scoring schemata.
Conference Talk at European Science Foundation - COST action: StatSeq meeting on RNA-Seq, Vienna, Austria
Comprehensive Evaluation of NGS Mapping Programs
Talk at Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
MASon: Million Alignments In Seconds - A Platform Independent Pairwise Sequence Alignment Library for Next Generation Sequencing Data.
Conference Talk at BIOINFORMATICS 2012, Vilamoura, Portugal
NextGenMap: Fast and accurate read mapping independent of evolutionary distance
Talk at Max Planck Institute for Evolutionary Biology, Ploen, Germany
NextGenMap: Fast and accurate read mapping independent of evolutionary distance.
Conference Talk at MASAMB 2013, London, UK
Conference Proceeding talk 3 on Relevant Topic in Your Field
Conference proceedings talk at Testing Institute of America 2014 Annual Conference, Los Angeles, CA
Impact of highly polymorphic regions on HTS analysis
Talk at University College London, London, UK
NextGenMap: How to accurately map 150,000 reads per second and why should we care
Talk at Cold Spring Harbor Laboratory, Cold Spring Harbor, USA
Methods for improved SV detection and interpretation.
Conference Talk at Genome in a bottle workshop, Gaithersburg, USA
Accurate Assessment of Structural Variations.
Talk at Cold Spring Harbor Laboratory, Cold Spring Harbor, USA
Structural Variation detection using long reads.
Conference Talk at Genome in a bottle workshop, Stanford, USA
Identification and interpretation of structural variations from yeast to human cancers using short and long reads.
Talk at University of Massachusetts, Worcester, USA
Identification of structural variations from long reads.
Talk at Johns Hopkins Univiersity, Baltimore, USA
Workshop on Structural Variations.
Organizer Workshop at Festival of Genomics, Boston, USA
Accurate detection of SVs using high throughput sequencing.
Talk at Cibiv-Max F. Perutz Laboratories, Vienna, Austria
Accurate and fast detection of complex and nested structural variations using long read technologies.
Talk at University of Lausanne, Lausanne, Switzerland
Accurate detection of SVs using short and long read sequencing.
Talk at Max Planck Institute for Evolutionary Biology, Ploen, Germany
Accurate detection and analysis of structural variations in healthy and cancerous human genomes.
Talk at University College London, London, UK
Accurate and fast detection of complex and nested structural variations using long read technologies.
Conference Talk at Biological Data Science, Cold Spring Harbor, USA
Identification and interpretation of structural variations from yeast to human cancers using short and long reads.
Talk at Baylor College of Medicine, Houston, USA
Mapping of NGS reads.
Talk at Johns Hopkins University, Baltimore, USA
Accurate detection of complex structural variations using PacBio.
Keynote Talk at PacBio Bioinformatics Workshop, Baltimore, USA
Structural Variation Characterization Across the Human Genome and Populations.
Talk at GRC/GiaB Workshop ASHG 2017, Orlando, USA
Accurate and Fast Detection of Complex and Nested Structural Variations Using Long-read Technologies.
Talk at ASHG 2017, Orlando, USA
Accurate and Fast Detection of Complex and Nested Structural Variations Using Long-read Technologies.
Talk at Functional Genomics: Towards Precision Medicine 2017, Doha, Qatar
Assessment of GiaB calls version 0.5.0.
Talk at GiaB Workshop, Standord, USA
CCDG Technology Development: Comprehensive Genomes
Talk at CCDG Workshop, Bethesda, USA
Size matters: accurate detection and phasing of Structural Variations
Talk at Radboud University Nijmegen Seminar, Nijmegen, Netherlands
Size matters: accurate detection and phasing of Structural Variations
Talk at SMRT Leiden conference, Leiden, Netherlands